PEPperCHIP® Pan-AAV Capsid Protein Microarray

Profile antibody responses against 15 AAV capsid proteins in one single assay.

Profile antibody responses against the most important wild type AAV capsid proteins with the PEPperCHIP® Pan-AAV Capsid Protein Microarray. The chip contains the sequences of 15 different capsid proteins converted into more than 5,000 overlapping peptides for high-resolution epitope data. Available with linear or cyclic constrained peptides for linear or conformational epitope mapping, the PEPperCHIP® Pan-AAV Capsid Protein Microarray can be used to analyze patient and animal sera as well as research and diagnostic anti-AAV antibodies on the epitope level.

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The PEPperCHIP® Pan-AAV Capsid Protein Microarray can be used for the following applications


Anti-AAV antibody monitoring in patient sera before and after gene therapy


Identification of cross-reacting anti-AAV antibodes


Identification of pre-existing anti-AAV antibody responses in healthy individuals


Fingerprint analysis of anti-AAV antibody responses in animal models


Validation of research or diagnostic antibodies against AAVs

Microarray information


Microarray content: Capsid proteins of AAV1 (protein ID NP_049542.1), AAV2 (protein ID P03135), AAV3b (protein ID O56139), AAV4 (protein ID O41855), AAV5 (protein ID Q9YIJ1), AAV6 (protein ID O56137), AAV7 (protein ID Q8JQG0), AAV8 (protein ID Q8JQF8), AAV9 (protein ID AAS99264.1), AAVrh.10 (protein ID AAO88201.1), AAVpo.1 (protein ID ACN42940.1), AAV10 (protein ID AAT46337.1), AAV11 (protein ID AAT46339.1), AAV12 (protein ID ABI16639.1) and AAV13 (protein ID ABZ10812)


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 13 aa (shift 2)


Number of peptides / spots:  Conformational microarray - 5,537 / 11,068 (peptides printed in duplicate); Linear microarray - 5,519 / 11,038 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) control peptides


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.




PEPperCHIP® Conformational Pan-AAV Capsid Protein Microarray Material Data Sheet [download pdf]


PEPperCHIP® Conformational Pan-AAV Capsid Protein Microarray Peptide Map [download xlsx]


PEPperCHIP® Linear Pan-AAV Capsid Protein Microarray Material Data Sheet [download pdf]


PEPperCHIP® Linear Pan-AAV Capsid Protein Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*
See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT

No scientific publications yet. Be the first one in publishing your data with this PEPperCHIP® Peptide Microarray.

PEPperCHIP® Pan-AAV Capsid Protein Microarray

Monitor antibody responses to AAV capsid proteins.

Identify, profile, and compare AAV-specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development, or screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research.

475.00 EUR

Excl. tax and shipping costs.

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