Identify epitope-level antibody responses against multiple human cytokines in a single assay with the PEPperCHIP® Human Cytokine Microarray. The peptide microarray contains 27 human cytokines, converted into more than 5,000 different peptides for high-resolution epitope analysis. Additionally included are HA and polio positive control peptides.
Screen patient sera or antibodies for autoimmune response monitoring, susceptibility to infectious diseases, or development of IVD and therapeutic antibodies.
The PEPperCHIP® Human Cytokine Microarray can be used for the following applications
• | Screening of patient sera for anti-human cytokine antibody responses with amino acid resolution. |
• | Identification and monitoring of autoimmune response development. |
• | Investigation of predisposition for infectious diseases. |
• | Identification and characterization of anti-human cytokine epitopes for IVD or therapeutic antibody development. |
Microarray information
• | Microarray content: human cytokines IL-1 alpha (UniProt ID P01583), IL-1 beta (UniProt ID P01584), IL-2 (UniProt ID P60568), IL-4 (UniProt ID P05112), IL-5 (UniProt ID P05113), IL-6 (UniProt ID P05231), IL-8 (CXCL8) (UniProt ID P10145), IL-10 (UniProt ID P22301), IL-12 subunit alpha (p35) (UniProt ID P29459), IL-12 subunit beta (p40) (UniProt ID P29460), IL-13 (UniProt ID P35225), IL-15 (UniProt ID P40933), IL-17A (UniProt ID Q16552), IL-18 (UniProt ID Q14116), IL-23A (UniProt ID Q9NPF7), IL-27 subunit alpha (UniProt ID Q8NEV9), IL-27 subunit beta (UniProt ID Q14213), IL-29 (IFN-lamda-1) (UniProt ID Q8IU54), IL-33 (UniProt ID O95760), TNF-alpha (UniProt ID P01375), TNF-beta (UniProt ID P01374), IFN-alpha-2 (UniProt ID P01563), IFN-beta (UniProt ID P01574), IFN-gamma (UniProt ID P01579), TGF-beta-1 (UniProt ID P01137), GM-CSF (UniProt ID P04141), and IP-10 (CXCL10) (UniProt ID P02778) |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) |
• | Number of peptides / spots: 5,414 / 10,828 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), and polio (KEVPALTAVETGAT) control peptides (50 and 44 spots respectively) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray. |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Human Cytokine Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Human Cytokine Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
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Screen purified antibodies, human, or animal sera for coronavirus-specific spike protein antigen recognition in one assay. Analyze the cross-reactivity of diagnostic and therapeutic antibodies, or compare patient-specific antibody response profiles for immunity or seroconversion monitoring.
475.00 EUR
Excl. tax and shipping costs.
