PEPperCHIP® Herpes-Simplex-Virus 1 Proteome Microarray

Profile antibody responses against the entire HSV-1 proteome.

The PEPperCHIP® Herpes-Simplex-Virus 1 Proteome Microarray covers all 74 HSV-1 proteins including all envelope glycoproteins converted into 19,408 different 15 amino acid peptides with a peptide overlap of 13 amino acids for high resolution epitope data. In addition, the microarray contains HA and polio positive control peptides.

Identify, profile and compare HSV-1 specific antibody responses on the epitope level for antibody biomarker discovery, antibody validation or vaccine development. Identify immunodominant anti-HSV-1 antibody responses or epitope signatures with a diagnostic or prognostic potential. Correlate antibody profiles with a potential role of HSV-1 in dementia or Alzheimer's disease. The microarray can be used for proteome-wide epitope mapping of anti-HSV-1 antibody responses, serum antibody profiling or biomarker discovery.

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Microarray information


Microarray content: 74 HSV-1 proteins including all envelope glycoproteins were converted into overlapping peptides


Peptide length and overlap: 15 aa peptides with an overlap of 13 aa (shift 2)


Number of peptides / spots: 19,4084 / 38,816 (peptides printed in duplicate)


Positive controls: HA (481 spots, YPYDVPDYAG) and polio (436 spots, KEVPALTAVETGAT) control peptides


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional accessories


6-8 weeks when requested with PEPperMAP® Services.



PEPperCHIP® Herpes-Simplex-Virus 1 Proteome Microarray Material Data Sheet [download pdf]


PEPperCHIP® Herpes-Simplex-Virus 1 Proteome Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



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PEPperCHIP® Neglected Tropical Diseases Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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