PEPperCHIP® Tumor Antigen Microarray

Profile antibody responses against 22 selected tumor-associated antigens.

The PEPperCHIP® Tumor Antigen Microarray covers 22 selected onco-antigens translated into 4,319 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 13-mer overlap (shift 2) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery.

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Microarray information


Microarray content: histone H1.2, histone H4, G2/mitotic-specific cyclin-B1, L-dopachrome tautomerase, cellular tumor antigen p53, receptor tyrosine-protein kinase erbB-2, mammaglobin A, TCRgamma alternate reading frame protein, stromelysin-3 (MMP11), protein SSX2 (Cancer/testis antigen 5.2), prostate-specific antigen, cancer/testis antigen 1 (NY-ESO-1), Myc proto-oncogene protein, claudin-6, carcinoembryonic antigen-related cell adhesion molecule 5, Melanocyte protein PMEL, Wilms tumor protein, tyrosinase, transcription factor SOX-2, myelin-oligodendrocyte glycoprotein, actin, alpha skeletal muscle, HLA class I histocompatibility antigen, A-36 alpha chain


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 13 aa (shift 2)


Number of peptides / spots: 4,319 / 8,638 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (28, 28 and 24 spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.



PEPperCHIP® Tumor Antigen Microarray Material Data Sheet [download pdf]


PEPperCHIP® Tumor Antigen Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



Discovery of Putative Breast Cancer Antigens Using an Integrative Platform of Genomics-Driven Immunoproteomics.
Veneta Qendro, Deborah H. Lundgren, Samuel Palczewski, Poornima Hegde, Christina Stevenson, Laurie Perpetua, Ardian Latifi, Jesse Merriman, Grace Bugos, and David K. Han. PROTEOMICS 17, no. 15–16 (August 2017): 1600318. []

Integrative Proteomics, Genomics, and Translational Immunology Approaches Reveal Mutated Forms of Proteolipid Protein 1 (PLP1) and Mutant-Specific Immune Response in Multiple Sclerosis.
Veneta Qendro, Grace A. Bugos, Debbie H. Lundgren, John Glynn, May H. Han, and David K. Han. PROTEOMICS 17, no. 6 (March 2017): 1600322. []

Genomics-Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases.
Raghavendra Giri, Veneta Qendro, Pooja Rani, Carren Jepchumba, Grace Bugos, Volker Stadler, and David K. Han. In Immunoproteomics, edited by Kelly M. Fulton and Susan M. Twine, 2024:327–32. New York, NY: Springer New York, 2019. []

PEPperCHIP® Tumor Antigen Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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