PEPperCHIP® Mouse Monoclonal anti-HA (12CA5)-Cy5 Control Antibody

For control staining of PEPperCHIP® Peptide Microarrays.

PEPperCHIP® Peptide Microarrays are printed with influenza virus hemagglutinin (HA) epitope peptides as built-in positive controls. They are usually arranged as a single spot frame around the main peptide content for each array copy. When visualized with immunofluorescence, these peptide spots help in aligning the .gal grid during data quantification. For optimal visualization, we recommend the use of our PEPperCHIP® anti-HA control antibodies for control staining.

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Product information

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Mouse monoclonal anti-HA control antibody 

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Fluorescent label: Cy5 for compatible microarray scanners (e.g., GenePix or Agilent)

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Volume: 10 µl at a stock concentration of 1 mg/ml

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Storage: 4oC or at -20oC for longer term 

 

Assay information

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A working dilution of 1:2000 is recommended

 

Estimated delivery time

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1-5 days after order confirmation

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1-4 weeks when requested with PEPperCHIP® Peptide Micorarrays

Learn how to perform the assay­­­

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PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]

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PEPperCHIP® Immunoassay Quickstart Guide [download pdf]

 

Steps

Materials and equipment

We recommend

Immunoassay

Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker

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Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies

Buffers

PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*
See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software

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*used in-house by PEPperPRINT

PEPperCHIP® Mouse Monoclonal anti-HA (12CA5)-Cy5 Control Antibody

Assay up to three peptide microarrays simultaneously.

<s>Take full control and perform PEPperCHIP® immunoassays at your own facility. Test multiple samples or multiple microarrays at once. Easy to assemble and fully reusable.</s>

<s>TBD.00 EUR</s>

<s>Excl. tax and shipping costs.</s>

<s>Request a Quote »</s>

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