Rockland Blocking Buffer (50 mL)

For superior detection of fluorescently labelled secondary antibodies with low background noise.

We highly recommend using Rockland blocking buffer (MB-070) for your PEPperCHIP® Peptide Microarray experiments. It blocks unspecific protein binding on the microarray, thereby effectively reducing background signals to generate high-quality data. Rockland blocking buffer is used for all PEPperMAP® Services.

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Product information

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Compatibility: can be used for blocking either linear or conformational PEPperCHIP® Peptide Micorarrays

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Volume: 50 mL (sterile filtered)

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Dilution: Optimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance

 

Storage information

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Store at 4oC prior to opening

! 

Do not freeze

 

Estimated delivery time

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1-5 days after order confirmation

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1-4 weeks when requested with PEPperCHIP® Peptide Micorarrays

Documentation

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Rockland Blocking Buffer Datasheet [download pdf]

 

Learn how to perform the assay­­­

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PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]

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PEPperCHIP® Immunoassay Quickstart Guide [download pdf]

 

Steps

Materials and equipment

We recommend

Immunoassay

Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker

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Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies

Buffers

PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*
See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software

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*used in-house by PEPperPRINT

Rockland Blocking Buffer (50 mL)

Assay up to three peptide microarrays simultaneously.

Take full control and perform PEPperCHIP® immunoassays at your own facility. Test multiple samples or multiple microarrays at once. Easy to assemble and fully reusable.

TBD.00 EUR

Excl. tax and shipping costs.

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