PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray

Profile antibody responses against the entire Tick-borne Encephalitis virus proteome.

The PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray covers the entire Tick-borne Encephalitis Virus translated into highly overlapping peptides. The microarray chip contains 3,414 linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

Identify, profile and compare Tick-borne Encephalitis Virus specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.

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Microarray information


Microarray content: Genome polyprotein of tick-borne encephalitis virus cleaved into 13 chains: peptide 2k, capsid protein C (core protein), prM, peptide pr, small envelope protein M (matrix protein), envelope protein E, non-structural protein 1, non-structural protein 2A, serine protease subunit NS2B (flavivirin protease NS2B regulatory subunit, non-structural protein 2B), serine protease NS3 (flavivirin protease NS3 catalytic subunit, non-structural protein 3), non-structural protein 4A, non-structural protein 4B, RNA-directed RNA polymerase NS5 (non-structural protein 5)


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1)


Number of peptides / spots: 3,414 / 6,828 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (37, 32 and 24 spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.



PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray Material Data Sheet [download pdf]


PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



Antibody Fingerprints in Lyme Disease Deciphered with High Density Peptide Arrays.
Laura K. Weber, Awale Isse, Simone Rentschler, Richard E. Kneusel, Andrea Palermo, Jürgen Hubbuch, Alexander Nesterov-Mueller, Frank Breitling, and Felix F. Loeffler. Engineering in Life Sciences 17, no. 10 (October 2017): 1078–87. [

PEPperCHIP® Tick-borne Encephalitis Virus Proteome Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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