PEPperCHIP® Signature Discovery Microarray

Discover unknown antigens or cross-reactive antibodies using a randomized library of 29,240 peptides.

The PEPperCHIP® Signature Discovery Microarray covers 29,240 randomized 15-mer linear peptides with varying amino acid distribution in duplicate. Moreover, the microarray contains HA, polio and c-Myc positive control peptides. The microarray can be used for epitope mapping, serum screening, antigen discovery as well as antibody cross-reactivity analysis.

Please note that this peptide microarray is only available in combination with our PEPperMAP® Services.

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Microarray information


Microarray content: 29,240 randomized peptides with the following amino acid frequencies: 6% for R, S, T, W, Y; 5% for A, D, E, F, G, H, I, K, L, M, N, P, Q, V


Peptide length: 15 aa


Number of Peptides / Spots: 29,240 / 58,480 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (261, 257 and 257 spots each control)


Size and format: Discovery Format (10.5 cm x 11 cm) containing 1 array copy per chip


Accessories: only available in combination with our PEPperMAP® Services 


Sample information


Can be assayed with at least 100 µL serum/plasma or 100 µg/mL purified antibody.


Estimated delivery time


6-8 weeks when requested with PEPperMAP® Services.




PEPperCHIP® Signature Discovery Microarray Material Data Sheet [download pdf]


PEPperCHIP® Signature Discovery Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



High-Throughput Epitope Profiling of Antibodies in the Plasma of Alzheimer’s Disease Patients Using Random Peptide Microarrays.
Kyu-Young Sim, Sang-Heon Park, Kyu Yeong Choi, Jung Eun Park, Jung Sup Lee, Byeong C. Kim, Jeonghwan Gwak, Woo Keun Song, Kun Ho Lee, and Sung-Gyoo Park. Scientific Reports 9, no. 1 (December 2019): 4587. [

PEPperCHIP® Signature Discovery Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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