PEPperCHIP® Oncovirus Epitope Microarray

Profile antibody responses against 3,653 B-cell epitopes associated with Oncoviruses.

The PEPperCHIP® Oncovirus Epitope Microarray covers 3,653 linear B-cell epitopes associated with human oncoviruses, including seven citrullinated peptides and the corresponding arginine variants. The peptide library contains 4,463 different 15-mer peptides printed in duplicates. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery.

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Microarray information

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Microarray content: 3,653 linear B-cell epitopes (3aa - 50 aa) of human oncoviruses from the Immune Epitope Database including 7 citrullinated peptides and the corresponding arginine variants.

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Microarray epitope lengths: 3 aa to 20 aa depending on the epitope length; longer epitopes were translated into overlapping 20 aa peptides

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Number of peptides / spots: 4,463 / 8,926 (peptides printed in duplicate)

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Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (40, 52 and 38 spots each control)

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Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip

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Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray

 

Sample information

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Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.

 

Estimated delivery time

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1-4 weeks for chip and optional add-on accessories

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6-8 weeks when requested with PEPperMAP® Services.

 

Documentation

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PEPperCHIP® Oncovirus Epitope Microarray Material Data Sheet [download pdf]

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PEPperCHIP® Oncovirus Epitope Microarray Peptide Map [download xlsx]

 

Learn how to perform the assay­­­

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PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]

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PEPperCHIP® Immunoassay Quickstart Guide [download pdf]

 

Steps

Materials and equipment

We recommend

Immunoassay

Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker

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Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies

Buffers

PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software

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*used in-house by PEPperPRINT

 

 

Discovery of Putative Breast Cancer Antigens Using an Integrative Platform of Genomics-Driven Immunoproteomics.
Veneta Qendro, Deborah H. Lundgren, Samuel Palczewski, Poornima Hegde, Christina Stevenson, Laurie Perpetua, Ardian Latifi, Jesse Merriman, Grace Bugos, and David K. Han. PROTEOMICS 17, no. 15–16 (August 2017): 1600318. [doi.org/10.1002/pmic.201600318]

Integrative Proteomics, Genomics, and Translational Immunology Approaches Reveal Mutated Forms of Proteolipid Protein 1 (PLP1) and Mutant-Specific Immune Response in Multiple Sclerosis.
Veneta Qendro, Grace A. Bugos, Debbie H. Lundgren, John Glynn, May H. Han, and David K. Han. PROTEOMICS 17, no. 6 (March 2017): 1600322. [doi.org/10.1002/pmic.201600322]

Genomics-Driven Immunoproteomics: An Integrative Platform to Uncover Important Biomarkers for Human Diseases.
Raghavendra Giri, Veneta Qendro, Pooja Rani, Carren Jepchumba, Grace Bugos, Volker Stadler, and David K. Han. In Immunoproteomics, edited by Kelly M. Fulton and Susan M. Twine, 2024:327–32. New York, NY: Springer New York, 2019. [doi.org/10.1007/978-1-4939-9597-4_21

PEPperCHIP® Oncovirus Epitope Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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