PEPperCHIP® Mycobacterium Tuberculosis Antigen Microarray

Profile antibody responses against 13 selected antigens of Mycobacterium Tuberculosis.

The PEPperCHIP® Mycobacterium Tuberculosis Antigen Microarray covers 13 selected antigens of Mycobacterium tuberculosis translated into 3,751 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery.

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Microarray information


Microarray content: 13 selected antigens of M. tuberculosis: ESX-1 secretion-associated protein, espB (Rv3881c), antigen 85-A, fbpA (Rv3804c), ESAT-6-like protein, esxB (Rv3874), alanine and proline-rich secreted protein, apa (Rv1860), lipoprotein, lprG (Rv1411c), alpha-crystallin, hspX (Rv2031c), phosphate-binding protein, pstS1 (Rv0934), ESX-1 secretion-associated protein, espA (Rv3616c), ESX-1 secretion-associated protein, espE (Rv3864), immunogenic protein, mpt64 (Rv1980c), enoyl-CoA hydratase, echA3 (Rv0632c), probable cutinase, cfp21 (Rv1984c), cell surface lipoprotein, mpt83 (Rv2873)


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1) 


Number of peptides / spots: 3,751 / 7,502 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (37, 32 and 26 and spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.




PEPperCHIP® Mycobacterium Tuberculosis Antigen Microarray Material Data Sheet [download pdf]


PEPperCHIP® Mycobacterium Tuberculosis Antigen Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



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PEPperCHIP® Mycobacterium Tuberculosis Antigen Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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