PEPperCHIP® Influenza Virus H1N1 Proteome Microarray

Profile antibody responses against the entire Influenza virus H1N1 proteome.

The PEPperCHIP® Influenza A Virus H1N1 Proteome Microarray covers 11 selected antigens from three different strains of Influenza A Virus H1N1 translated into 4,856 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 13-mer overlap (shift 2) in duplicates for full epitope coverage. Moreover, the microarray contains HA and polio positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery.

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Microarray information


Microarray content: A: Hemagglutinin, B: Matrix protein 1, C: Matrix protein 2, D: Nucleoprotein, E: Nuclear export protein, F: Neuraminidase, G: Non-structural protein 1, H: Polymerase acidic protein, I: Protein PB1-F2, J: Polymerase basic protein 2, K: RNA-directed RNA polymerase catalytic subunit


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 13 aa (shift 2)


Number of peptides / spots: 4,856 / 9,712 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) control peptides (108 spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.



PEPperCHIP® Influenza Virus H1N1 Proteome Microarray Material Data Sheet [download pdf]


PEPperCHIP® Influenza Virus H1N1 Proteome Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



Immunity to Influenza Is Dependent on MHC II Polymorphism: Study with 2 HLA Transgenic Strains.
David Luckey, Eric A. Weaver, Douglas G. Osborne, Daniel D. Billadeau, and Veena Taneja. Scientific Reports 9, no. 1 (December 2019): 19061. [

Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine.
Amy Lingel, Brianna L. Bullard, and Eric A. Weaver. Scientific Reports 7, no. 1 (December 2017): 14912. [

Identification of a Highly Conserved Epitope on Avian Influenza Virus Non-Structural Protein 1 Using a Peptide Microarray.
Jiashan Sun, Xiurong Wang, Xuexia Wen, Hongmei Bao, Lin Shi, Qimeng Tao, Yongping Jiang, et al. Edited by Qianjun Li. PLOS ONE 11, no. 3 (March 3, 2016): e0149868. []

PEPperCHIP® Influenza Virus H1N1 Proteome Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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