The PEPperCHIP® Influenza A Virus H1N1 Proteome Microarray covers 11 selected antigens from three different strains of Influenza A Virus H1N1 translated into 4,856 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 13-mer overlap (shift 2) in duplicates for full epitope coverage. Moreover, the microarray contains HA and polio positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: A: Hemagglutinin, B: Matrix protein 1, C: Matrix protein 2, D: Nucleoprotein, E: Nuclear export protein, F: Neuraminidase, G: Non-structural protein 1, H: Polymerase acidic protein, I: Protein PB1-F2, J: Polymerase basic protein 2, K: RNA-directed RNA polymerase catalytic subunit |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 13 aa (shift 2) |
• | Number of peptides / spots: 4,856 / 9,712 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) control peptides (108 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Influenza Virus H1N1 Proteome Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Influenza Virus H1N1 Proteome Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Immunity to Influenza Is Dependent on MHC II Polymorphism: Study with 2 HLA Transgenic Strains.
David Luckey, Eric A. Weaver, Douglas G. Osborne, Daniel D. Billadeau, and Veena Taneja. Scientific Reports 9, no. 1 (December 2019): 19061. [doi.org/10.1038/s41598-019-55503-1]
Efficacy of an Adenoviral Vectored Multivalent Centralized Influenza Vaccine.
Amy Lingel, Brianna L. Bullard, and Eric A. Weaver. Scientific Reports 7, no. 1 (December 2017): 14912. [doi.org/10.1038/s41598-017-14891-y]
Identification of a Highly Conserved Epitope on Avian Influenza Virus Non-Structural Protein 1 Using a Peptide Microarray.
Jiashan Sun, Xiurong Wang, Xuexia Wen, Hongmei Bao, Lin Shi, Qimeng Tao, Yongping Jiang, et al. Edited by Qianjun Li. PLOS ONE 11, no. 3 (March 3, 2016): e0149868. [doi.org/10.1371/journal.pone.0149868]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
