PEPperCHIP® Infectious Disease Epitope Microarray

Profile antibody responses against 3,760 B-cell epitopes of infectious diseases.

The PEPperCHIP® Infectious Disease Epitope Microarray covers 3,760 linear B-cell epitopes associated with infectious diseases from 196 different pathogens. The peptide library contains 4,344 different 15-mer peptides printed in duplicates. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery.

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The PEPperCHIP® Infectious Disease Epitope Microarray can be used for the following applications


Identification of unknown infections in patient sera


Discovery of hidden secondary infections


Correlation of autoimmune diseases with antibody responses raised by infections


Immune monitoring of patients before and after infections and immunizations


Multiplexed analysis of IgG, IgM and IgA antibody responses

Microarray information


Microarray content: 3,760 linear B-cell epitopes of 196 different pathogens referenced in the Immune Epitope Database and two selected Toxoplasma gondii antigens as overlapping peptides.


Microarray epitope lengths: 4 aa - 17 aa depending on the database epitope length; longer epitopes were translated into overlapping 17 aa peptides


Number of peptides / spots: 4,344 / 8,688 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (40, 42 and 30 spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.




PEPperCHIP® Infectious Disease Epitope Microarray Material Data Sheet [download pdf]


PEPperCHIP® Infectious Disease Epitope Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



Identification of the Atypically Modified Autoantigen Ars2 as the Target of B-Cell Receptors from Activated B Cell–Type Diffuse Large B-Cell Lymphoma. 
Lorenz Thurner, Sylvia Hartmann, Moritz Bewarder, Natalie Fadle, Evi Regitz, Claudia Schormann, Natalia Quiroga, u. a. Haematologica, 16. Juli 2020, haematol.2019.241653. []

Lymphocyte Predominant Cells Detect Moraxella Catarrhalis-Derived Antigens in Nodular Lymphocyte-Predominant Hodgkin Lymphoma.
Lorenz Thurner, Sylvia Hartmann, Natalie Fadle, Evi Regitz, Maria Kemele, Yoo-Jin Kim, Rainer Maria Bohle, et al. Nature Communications 11, no. 1 (May 2020): 2465. [

LRPAP1 Is a Frequent Proliferation-Inducing Antigen of BCRs of Mantle Cell Lymphomas and Can Be Used for Specific Therapeutic Targeting.
Lorenz Thurner, Sylvia Hartmann, Natalie Fadle, Maria Kemele, Theresa Bock, Moritz Bewarder, Evi Regitz, et al. Leukemia 33, no. 1 (January 2019): 148–58. [

PEPperCHIP® Infectious Disease Epitope Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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