The PEPperCHIP® Infectious Disease Epitope Microarray covers 3,760 linear B-cell epitopes associated with infectious diseases from 196 different pathogens. The peptide library contains 4,344 different 15-mer peptides printed in duplicates. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
The PEPperCHIP® Infectious Disease Epitope Microarray can be used for the following applications
• | Identification of unknown infections in patient sera |
• | Discovery of hidden secondary infections |
• | Correlation of autoimmune diseases with antibody responses raised by infections |
• | Immune monitoring of patients before and after infections and immunizations |
• | Multiplexed analysis of IgG, IgM and IgA antibody responses |
Microarray information
• | Microarray content: 3,760 linear B-cell epitopes of 196 different pathogens referenced in the Immune Epitope Database and two selected Toxoplasma gondii antigens as overlapping peptides. |
• | Microarray epitope lengths: 4 aa - 17 aa depending on the database epitope length; longer epitopes were translated into overlapping 17 aa peptides |
• | Number of peptides / spots: 4,344 / 8,688 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (40, 42 and 30 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Infectious Disease Epitope Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Infectious Disease Epitope Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Identification of the Atypically Modified Autoantigen Ars2 as the Target of B-Cell Receptors from Activated B Cell–Type Diffuse Large B-Cell Lymphoma.
Lorenz Thurner, Sylvia Hartmann, Moritz Bewarder, Natalie Fadle, Evi Regitz, Claudia Schormann, Natalia Quiroga, u. a. Haematologica, 16. Juli 2020, haematol.2019.241653. [doi.org/10.3324/haematol.2019.241653]
Lymphocyte Predominant Cells Detect Moraxella Catarrhalis-Derived Antigens in Nodular Lymphocyte-Predominant Hodgkin Lymphoma.
Lorenz Thurner, Sylvia Hartmann, Natalie Fadle, Evi Regitz, Maria Kemele, Yoo-Jin Kim, Rainer Maria Bohle, et al. Nature Communications 11, no. 1 (May 2020): 2465. [doi.org/10.1038/s41467-020-16375-6]
LRPAP1 Is a Frequent Proliferation-Inducing Antigen of BCRs of Mantle Cell Lymphomas and Can Be Used for Specific Therapeutic Targeting.
Lorenz Thurner, Sylvia Hartmann, Natalie Fadle, Maria Kemele, Theresa Bock, Moritz Bewarder, Evi Regitz, et al. Leukemia 33, no. 1 (January 2019): 148–58. [doi.org/10.1038/s41375-018-0182-1]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
