PEPperCHIP® Human Epitome Microarray

Discover unknown human antigens or cross-reactive antibodies by screening 29,128 human B-cell epitopes.

The PEPperCHIP® Human Epitome Microarray covers 29,128 linear B-cell epitopes of the Immune Epitope Database. The peptide library contains selected epitopes of 3,209 different proteins from 591 different organisms. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

The PEPperCHIP® Human Epitome Microarray can be used to investigate links between autoimmune and infectious diseases. The microarray can be also used for epitope mapping, serum screening, biomarker discovery and cross-reactivity analysis of therapeutic and diagnostic antibodies.

Due to the discovery microarray format and the resulting large data sets, PEPperCHIP® Human Epitome Microarrays are exclusively provided in combination with our PEPperMAP® Services.

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The PEPperCHIP® Human Epitome Microarray enables the high throughput screening of serum and plasma antibody responses against all linear human B-cell epitopes of the Immune Epitope Database: This corresponds to a 29,128-peptide ELISA in a single assay with less than 100 µL sample required.

The PEPperCHIP® Human Epitome Microarray can be used for the following applications

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Epitome-wide antibody screening

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Differentiation between IgG and IgM or IgA response profiles

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High throughput serum biomarker discovery

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Identification of prognostic epitopes

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Cross-reactivity analysis of mono- and polyclonal antibodies

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Patient stratification

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Investigation of links between autoimmune and infectious diseases

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Cross-reactivity analysis of therapeutic and diagnostic antibodies

 

See also

Microarray information

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Microarray content: Sorted by disease states, the microarray covers B-cell epitopes selected from the Immune Epitope Database of the following indications:
 
- 8,504 epitopes of infectious diseases
- 3,808 epitopes of autoimmune diseases including 301 citrullinated peptides
- 3,451 epitopes of allergens
- 64 epitopes of transparent diseases
- 199 cancer epitopes
- 995 epitopes of other diseases
- 1,481 epitopes of healthy controls
- 4,661 epitopes of the most common vaccines

 

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Database epitope lengths: 5 aa - 51 aa

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Microarray epitope lengths:  5 to 17 aa depending on the epitope length; longer epitopes were translated into overlapping 17 aa peptides

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Number of peptides / spots: 29,128 / 58,256 (peptides printed in duplicate)

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Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (261, 257 and 257 spots each control)

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Size and format: Discovery Format (10.5 cm x 11 cm) containing 1 array copy per chip

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Accessories: only available in combination with our PEPperMAP® Services 

 

Sample information

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Can be assayed with at least 100 µL serum/plasma or 100 µg/mL purified antibody.

 

Estimated delivery time

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6-8 weeks when requested with PEPperMAP® Services.

Documentation

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PEPperCHIP® Human Epitome Microarray Material Data Sheet [download pdf]

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PEPperCHIP® Human Epitome Microarray Peptide Map [download xlsx]

 

Learn how to perform the assay­­­

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PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]

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PEPperCHIP® Immunoassay Quickstart Guide [download pdf]

 

Steps

Materials and equipment

We recommend

Immunoassay

Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker

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Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies

Buffers

PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software

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*used in-house by PEPperPRINT

 

 

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PEPperCHIP® Human Epitome Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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