PEPperCHIP® HIV-1 (Env) Antigen Microarray

Profile antibody responses against HIV-1 envelope protein gp160.

The PEPperCHIP® HIV-1 (Env) Antigen Microarray covers HIV-1 envelope protein gp160 (gp120 + gp41) translated into 856 highly overlapping linear peptides. The microarray chip contains linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Each microarray chip contains three array copies for one-by-one assays. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery.

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Microarray information


Microarray content: Envelope glycoprotein (Env) gp160 of HIV type 1 cleaved into 2 chains: Surface protein gp120 and Transmembrane protein gp41


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1)


Number of peptides / spots: 856 / 1,712 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (22, 28 and 20 spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/3 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.



PEPperCHIP® HIV-1 (Env) Antigen Microarray Material Data Sheet [download pdf]


PEPperCHIP® HIV-1 (Env) Antigen Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



HIV-1 Gp140 Epitope Recognition Is Influenced by Immunoglobulin DH Gene Segment Sequence.
Yuge Wang, Pratibha Kapoor, Robert Parks, Aaron Silva-Sanchez, S. Munir Alam, Laurent Verkoczy, Hua-Xin Liao, et al. Immunogenetics 68, no. 2 (February 2016): 145–55. [

PEPperCHIP® HIV-1 (Env) Antigen Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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