The PEPperCHIP® HCV Proteome Microarray covers the entire HCV proteome translated into overlapping peptides. The microarray chip contains 3,084 linear 15-mer peptides with a 12-mer overlap (shift 3) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
Identify, profile and compare HCV specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.
Microarray information
• | Microarray content: Genome polyprotein of hepatitis C virus cleaved into 11 chains: core protein p21 (Capsid protein C, p21), core protein p19, envelope glycoprotein E1 (gp32, gp35), envelope glycoprotein E2 (NS1, gp68, gp70), p7, Protease NS2-3 (p23), serine protease NS3 (Hepacivirin, NS3P, p70), non-structural protein 4A (NS4A, p8), Non-structural protein 4B (NS4B, p27), Non-structural protein 5A (NS5A, p56), RNA-directed RNA polymerase (NS5B, p68) |
• | Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 12 aa (shift 3) |
• | Number of peptides / spots: 3,084 / 6,168 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (31, 32 and 22 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® HCV Proteome Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® HCV Proteome Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Development and Characterization of a Human Monoclonal Antibody Targeting the N-Terminal Region of Hepatitis C Virus Envelope Glycoprotein E1.
Ahmed Atef Mesalam, Isabelle Desombere, Ali Farhoudi, Freya Van Houtte, Lieven Verhoye, Jonathan Ball, Jean Dubuisson, et al. Virology 514 (January 2018): 30–41. [doi.org/10.1016/j.virol.2017.10.019]
A Novel Neutralizing Human Monoclonal Antibody Broadly Abrogates Hepatitis C Virus Infection in Vitro and in Vivo.
Isabelle Desombere, Ahmed Atef Mesalam, Richard A. Urbanowicz, Freya Van Houtte, Lieven Verhoye, Zhen-Yong Keck, Ali Farhoudi, et al. Antiviral Research 148 (December 2017): 53–64. [doi.org/10.1016/j.antiviral.2017.10.015]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
