PEPperCHIP® HBV Proteome Microarray

Profile antibody responses against the entire HBV proteome.

The PEPperCHIP® HBV Proteome Microarray covers the entire HBV proteome translated into overlapping peptides. The microarray chip contains 4,731 linear 15-mer peptides with a 12-mer overlap (shift 3) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

Identify, profile and compare HBV specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.

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Microarray information


Microarray content: Proteomes of all different genotypes, and various subtypes, of HBV; linked and elongated with neutral GSGSGSG linkers at the C- and N-terminus and translated into overlapping peptides. Redundant peptides were removed.


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 12 aa (shift 3)


Number of peptides / spots: 4,713 / 9,426 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (28, 36 and 24 spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.




PEPperCHIP® HBV Proteome Microarray Material Data Sheet [download pdf]


PEPperCHIP® HBV Proteome Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



Predicting HBsAg Clearance in Genotype A Chronic Hepatitis B Using HBsAg Epitope Profiling: A Biomarker for Functional Cure.
Renae Walsh, Rachel Hammond, Lilly Yuen, Joshua Deerain, Tanya O’Donnell, Thomas Leary, Gavin Cloherty, et al. Liver International 39, no. 11 (November 2019): 2066–76. []

PEPperCHIP® HBV Proteome Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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