PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray

Profile antibody responses against the entire Foot-and-mouth Disease virus.

The PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray covers the entire foot-and-mouth disease virus proteome translated into highly overlapping peptides. The microarray chip contains 2,332 linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

Identify, profile and compare Foot-and-mouth Disease Virus specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.

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Microarray information


Microarray content: Genome polyprotein of foot-and-mouth disease virus cleaved into 15 chains: Leader protease (Lpro), protein VP0 (VP4-VP2), protein VP4 (P1A, Virion protein 4), protein VP2 (P1B, Virion protein 2), protein VP3 (P1C, Virion protein 3), protein VP1 (P1D, Virion protein 1), protein 2A (P52), Protein 2B, protein 2C, protein 3A, protein 3B-1 (Genome-linked protein VPg1), protein 3B-2 (Genome-linked protein VPg2), protein 3B-3 (Genome-linked protein VPg3), picornain 3C (Protease 3C, Protease P20B), RNA-directed RNA polymerase 3D-POL (P56A)


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1)


Number of peptides / spots: 2,332 / 4,664 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (28, 36 and 26 spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.




PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray Material Data Sheet [download pdf]


PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



Universal Detection of Foot and Mouth Disease Virus Based on the Conserved VP0 Protein.
Silvia Loureiro, Claudine Porta, Hemanta K. Maity, Eva Perez, Flavia F. Bagno, Abhay Kotecha, Elizabeth Fry, et al. Wellcome Open Research 3 (July 25, 2018): 88. [

PEPperCHIP® Foot-and-mouth Disease Virus Proteome Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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