PEPperCHIP® Cyclic Citrullinated Peptide Microarray

Selectively screen for anti-CCP autoantibody responses.

The PEPperCHIP® Cyclic Citrullinated Peptide Microarray covers 577 different peptides printed in duplicate. The microarray chip contains 337 different cyclic citrullinated peptides and the corresponding arginine variants. Peptide cyclization has been achieved by thioether cyclization between C-terminal cysteine and modified N-terminus. The microarray chip contains five array copies for one-by-one assays including HA, polio and c-Myc positive control peptides.

The microarray can be used epitope mapping, anti-CCP serum screening or biomarker discovery of autoantigens.

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Microarray information


Microarray content: 337 cyclic citrullinated peptides of the Immune Epitope Database and from literature including the corresponding arginine variants.


Peptide length and overlap: 10 aa - 17 aa cyclic constrained peptides via N- to C-terminal thioether formation


Number of peptides / spots: 577 / 1,154 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (26, 22 and 22 spots each control)


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 5 array copies per chip


Accessories: compatible with 3/5 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.




PEPperCHIP® Cyclic Citrullinated Peptide Microarray Material Data Sheet [download pdf]


PEPperCHIP® Cyclic Citrullinated Peptide Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



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PEPperCHIP® Cyclic Citrullinated Peptide Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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