PEPperCHIP® CNS Peptide Microarray

Profile antibody responses associated with neuroinflammatory disorders with amino acid resolution.

The PEPperCHIP® CNS Peptide Microarray covers 25 proteins associated with neuroinflammatory disorders (including several myelin proteins, aquaporin-4, alpha-synuclein or amyloid beta precursor protein) converted into 5,573 different overlapping peptides. The microarray contains linear 15-mer peptides with a peptide-peptide overlap of 13 amino acids for high resolution epitope data. Moreover, the microarray contains additional HA and polio positive control peptides.

The PEPperCHIP® CNS Peptide Microarray can be used to:

  • Discriminate antibody responses between different inflammatory diseases of the nervous system such as multiple sclerosis, Alzheimer’s disease or encephalomyelitis
  • Identification of pathogenic autoantibodies as potential biomarkers in patients with suspected autoimmune neurologic disorders
  • Differentiation of neural tissue-specific Ig responses via testing serum and CSF simultaneously
  • Monitoring of patients treated with emerging therapies.

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Microarray information


Microarray content: 25 proteins associated with neuroinflammatory disorders 


Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 13 aa (shift 2)


Number of peptides / spots: 5,573 / 11,146 (peptides printed in duplicate)


Positive control: HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) control peptides


Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip


Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray


Sample information


Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.


Estimated delivery time


1-4 weeks for chip and optional add-on accessories


6-8 weeks when requested with PEPperMAP® Services.




PEPperCHIP® CNS Peptide Microarray Material Data Sheet [download pdf]


PEPperCHIP® CNS Peptide Microarray Peptide Map [download xlsx]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT



No scientific publications yet. Be the first one in publishing your data with this PEPperCHIP® Peptide Microarray.

PEPperCHIP® Transglutaminase Peptide Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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