PEPperCHIP® African Swine Fever Virus Microarray
Profile antibody responses against 12 selected ASVF antigens.
The PEPperCHIP® African Swine Fever Virus Microarray covers 12 selected antigens from African Swine Fever Virus translated into 5,410 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA and c-Myc positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
Microarray content: 12 selected antigens of African swine fever virus (strain Badajoz 1971 Vero-adapted): Attachment protein p12, Cd2-like protein, B646L, E120R, B438L, Envelope protein p54, D117L, Protein E248R, BA71V-CP2475L (P220), Polyprotein pp62, Cysteine protease S273R and BA71V-K78R (P10)
Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1)
Number of peptides / spots: 5,410 / 10,820 (peptides printed in duplicate)
Positive control: HA (YPYDVPDYAG) and c-Myc (EQKLISEEDL) control peptides (116 and 116 spots each control)
Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip
Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray
Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.
Estimated delivery time
1-4 weeks for chip and optional add-on accessories
6-8 weeks when requested with PEPperMAP® Services.
PEPperCHIP® Epstein-Barr Virus Antigen Microarray Material Data Sheet [download pdf]
PEPperCHIP® Epstein-Barr Virus Antigen Microarray Peptide Map [download xlsx]
Learn how to perform the assay
PEPperCHIP® Immunoassay Quickstart Guide [download pdf]
Materials and equipment
PEPperCHIP® Incubation Tray*
BSA, or Rockland Blocking Buffer*
PEPperCHIP® Control Antibodies
PBS, Tween 20
Labelled secondary antibodies
Rockland secondary antibodies*
Innopsys InnoScan® 710/710-IR*
Fluorescence spot intensity quantification software
MAPIX Analyzer by Innopsys
*used in-house by PEPperPRINT
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