PEPperCHIP® African Swine Fever Virus Microarray

Profile antibody responses against 12 selected ASVF antigens.

The PEPperCHIP® African Swine Fever Virus Microarray covers 12 selected antigens from African Swine Fever Virus translated into 5,410 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA and c-Myc positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery.

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Microarray information

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Microarray content: 12 selected antigens of African swine fever virus (strain Badajoz 1971 Vero-adapted): Attachment protein p12, Cd2-like protein, B646L, E120R, B438L, Envelope protein p54, D117L, Protein E248R, BA71V-CP2475L (P220), Polyprotein pp62, Cysteine protease S273R and BA71V-K78R (P10)

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Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1)

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Number of peptides / spots: 5,410 / 10,820 (peptides printed in duplicate)

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Positive control: HA (YPYDVPDYAG) and c-Myc (EQKLISEEDL) control peptides (116 and 116 spots each control)

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Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip

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Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray

 

Sample information

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Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.

 

Estimated delivery time

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1-4 weeks for chip and optional add-on accessories

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6-8 weeks when requested with PEPperMAP® Services.

Documentation

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PEPperCHIP® Epstein-Barr Virus Antigen Microarray Material Data Sheet [download pdf]

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PEPperCHIP® Epstein-Barr Virus Antigen Microarray Peptide Map [download xlsx]

 

Learn how to perform the assay­­­

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PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]

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PEPperCHIP® Immunoassay Quickstart Guide [download pdf]

 

Steps

Materials and equipment

We recommend

Immunoassay

Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker

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Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies

Buffers

PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software

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*used in-house by PEPperPRINT

 

 

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PEPperCHIP® African Swine Fever Virus Microarray

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