Rockland Blocking Buffer (500 mL)

For superior detection of fluorescently labelled secondary antibodies with low background noise.

We highly recommend using Rockland blocking buffer (MB-070) for your PEPperCHIP® Peptide Microarray experiments. It blocks unspecific protein binding on the microarray, thereby effectively reducing background signals to generate high-quality data. Rockland blocking buffer is used for all PEPperMAP® Services.

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Product information


Compatibility: can be used for blocking either linear or conformational PEPperCHIP® Peptide Micorarrays


Volume: 500 mL (sterile filtered)


Dilution: Optimum performance is achieved using this product undiluted. However, this blocking solution can often be diluted 1:1 in TBS without a significant loss of performance


Storage information


Store at 4oC prior to opening


Do not freeze


Estimated delivery time


1-5 days after order confirmation


1-4 weeks when requested with PEPperCHIP® Peptide Micorarrays



Rockland Blocking Buffer Datasheet [download pdf]


Learn how to perform the assay­­­


PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]


PEPperCHIP® Immunoassay Quickstart Guide [download pdf]



Materials and equipment

We recommend


Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker


Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies


PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*
See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software


*used in-house by PEPperPRINT

Rockland Blocking Buffer (500 mL)

Assay up to three peptide microarrays simultaneously.

Take full control and perform PEPperCHIP® immunoassays at your own facility. Test multiple samples or multiple microarrays at once. Easy to assemble and fully reusable.


Excl. tax and shipping costs.

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