PEPperCHIP® Dengue Virus Type 1 Proteome Microarray

Profile antibody responses against the entire Dengue virus type 1 proteome.

The PEPperCHIP® Dengue Virus Type 1 Proteome Microarray covers the entire Dengue Type 1 viral proteome translated into highly overlapping peptides. The microarray chip contains 3,396 linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

Identify, profile and compare Dengue Virus specific IgG, IgA, and IgM responses in patient or animal sera for vaccine development. Screen viral antigens to find and characterize immunodominant epitopes for in-vitro diagnostics research. The microarray can be used for proteome-wide epitope mapping, serum screening or biomarker discovery.

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Microarray information

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Microarray content: Genome polyprotein (strain Singapore/S275/1990, UniProt ID P33478) translated into overlapping peptides

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Peptide length and overlap:  15 aa peptides with peptide-peptide overlap of 14 aa (shift 1)

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Number of peptides / spots: 3,396 / 6,792 (peptides printed in duplicate)

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Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (44, 36 and 26 spots each control)

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Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip

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Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray

 

Sample information

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Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.

 

Estimated delivery time

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1-4 weeks for chip and optional add-on accessories

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6-8 weeks when requested with PEPperMAP® Services.

 

Documentation

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PEPperCHIP® Dengue Virus Type 1 Proteome Microarray Material Data Sheet [download pdf]

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PEPperCHIP® Dengue Virus Type 1 Proteome Microarray Peptide Map [download xlsx]

 

Learn how to perform the assay­­­

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PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]

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PEPperCHIP® Immunoassay Quickstart Guide [download pdf]

 

Steps

Materials and equipment

We recommend

Immunoassay

Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker

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Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies

Buffers

PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software

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*used in-house by PEPperPRINT

 

 

Mapping Putative B-Cell Zika Virus NS1 Epitopes Provides Molecular Basis for Anti-NS1 Antibody Discrimination between Zika and Dengue Viruses.
Marjorie C. L. C. Freire, Laércio Pol-Fachin, Danilo F. Coêlho, Isabelle F. T. Viana, Tereza Magalhães, Marli T. Cordeiro, Nico Fischer, et al. ACS Omega 2, no. 7 (July 31, 2017): 3913–20. [doi.org/10.1021/acsomega.7b00608]

PEPperCHIP® Dengue Virus Type 1 Proteome Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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