Presented by: Jill Roughan, PhD, Technical Sales Representative of PEPperPRINT GmbH, San Diego, USA
Date: September 7, 2016
Antibodies are essential life science tools for therapeutics, basic research and diagnostic tests. However, mono- and polyclonal antibodies are often poorly characterized in terms of specificity and cross-reactivity, as summarized in the recent Nature Feature Reproducibility crisis: Blame it on the antibodies. The Human Protein Atlas consortium from Sweden showed that less than 50% of commercially available antibodies can be effectively used for immunohistochemical tests, which underlines the urgent need for antibody validation and cross-reactivity testing.
Based on its laser printed high-density peptide microarrays, PEPperPRINT developed a comprehensive toolbox for antibody validation and cross-reactivity analysis. This toolbox comprises solutions for linear and conformational epitope mappings, epitope substitution scans for the identification of conserved and variable amino acid positions for antibody binding, and robust cross-reactivity screening with the new PEPperCHIP® Human Epitome Microarray. To learn more about PEPperPRINT’s toolbox for accurate, fast and cost effective antibody characterization and validation, we invite you to watch our scientific webinar.
About the Presenter
Dr. Jill Roughan joined PEPperPRINT in April 2016 as technical sales representative on the West Coast, USA. For over 10 years prior, she has worked for research institutes, small start-up companies and large biotech and medical device corporations as an independent researcher, product and marketing manager. Dr. Roughan has managed small product portfolios but also large ones comprising up to 5000 products sold nationally and internationally.
Prior to her time at The Scripps Research Institute studying B cell responses in HIV and HCV vaccine design, she received her PhD in Immunology at Tufts Medical School in Boston. She is an author of numerous peer-reviewed journal articles and received scholarships and grants fully supporting her educational and research career.