High Resolution Linear Epitope Mapping

PEPperCHIP® Peptide Microarrays enable the high resolution epitope mapping of antibodies and sera by translating one or more antigens into overlapping peptides. Incubation of the resulting peptide microarray with an antibody or serum sample as well as suited secondary antibodies gives rise to spot patterns that correlate with the epitope of the given sample.


Due to the very high spot density and the widely unlimited peptide content flexibility, PEPperCHIP® Peptide Microarrays are the ideal and most economic solution to identify antibody and serum epitopes in the highest resolution possible. 


High resolution epitope mappings can be based on e.g. a single antigen using PEPperCHIP® Custom Peptide Microarrays with standard dimensions or on hundreds of proteins at once using large scale PEPperCHIP® Discovery Microarrays.

 

Specifications of PEPperMAP® Linear Epitope Mapping

The PEPperMAP® Linear Epitope Mapping is the standard epitope mapping format based on a single peptide length and the maximum peptide overlap for high resolution epitope data. The PEPperMAP® Linear Epitope Mapping is the recommended solution for monoclonal antibodies and serum samples.

Type 1 Epitope Mapping

PEPperMAP® Linear Epitope Mapping of a monoclonal antibody against an antigen translated into 15 aa peptides with 14 aa peptide overlap. The microarray scan (left) as well as the intensity plot (right) reveal a stretch of adjacent peptides with a consensus motif that represents the epitope of the antibody. Stained HA (red) and Flag (green) control peptides frame the antigen-derived peptides.

   Microarray Content:   Single to multiple antigens translated into overlapping peptides
   Peptide Length:   15 amino acids by default (adjustable)
   Peptide Overlap:   14 amino acids by default (adjustable)
   Peptide Repeats:   Peptide double spots by default (adjustable)
   Control Peptides:   HA (YPYDVPDYAG) epitopes by default
   Microarray Example:   A protein sequence of 400 amino acids will be translated into 2 x 400 different peptides

 

Input Data & Sample Material

  • Protein sequence(s) in the FASTA format or with UniProt ID
  • 10-20 µl serum or 10-20 µg purified antibody



Analysis

PEPperCHIP® Peptide Microarray analysis is straightforward and can be performed in your own lab or covered by a PEPperMAP® Service. PEPperMAP® Linear Epitope Mappings are based on cost effective flat-rate fees. Information about lab requirements for PEPperCHIP® Peptide Microarray analysis can be found here.

Further Information

 

 

 

 

 

 

 

 

 

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