PEPperCHIP® handling and analysis is straightforward and follows standard immunoassay protocols. To get a first glance about working with PEPperCHIP® Peptide Microarrays, we recommend to watch the short tutorial movie. More details are available in our standard immunoassay protocol. Typical lab and material requirements for PEPperCHIP® Peptide Microarray assays are listed below.
Please note that PEPperPRINT also offers a PEPperCHIP® Training Kit to practice peptide microarray handling and analysis. The comprehensive PEPperMAP® Services further enable you to completely outsource immunoassays, data read-out and quantification to PEPperPRINT's peptide microarray experts.
"We were working with the PEPperCHIP for the first time in order to map epitopes in the envelope protein of a new emerging virus. The provided protocol was easy to perform and staining with the recommended antibodies resulted in explicit results. The provided software PepSlide Analyser had all tools we needed for a detailed evaluation and was easy to use. Special thanks to the support team of PEPperPRINT. Every kind of question was answered sufficiently within a very short time."
(Robert Fux, PhD, Institute of Infectious Diseases and Zoonoses, Ludwig-Maximilians-University of Munich)
Material Provided by PEPperPRINT
- PEPperCHIP® Incubation Tray adjusted to the given microarray layout for one-by-one assays with multiple array copies per chip and low incubation volumes
- PEPperCHIP® Control Antibodies for staining of routinely implemented HA control peptides
- MAPIX Analyzer as optional well-adjusted software solution for peptide microarray image analysis
General Lab and Material Requirements
- Orbital shakers
- Microarray scanners: The PEPperCHIP® Peptide Microarrays are compatible with most microarray scanners (e.g. GenePix 4000B, 4100B and 4300/4400 Microarray Scanners, Tecan Laser and Power Scanner, Agilent High-Resolution and SureScan Microarray Scanner, Innopsys InnoScan 710 and 900, NimbleGen MS 200 Microarray Scanner, LI-COR Odyssey Imaging System and many more).
- Buffers: PBS, Tween
- Blocking reagents: BSA or Rockland Blocking Buffer
- Sample: 10-20 µl serum/plasma or 20 µg purified antibody
- Labeled secondary antibody for staining of primary samples(e.g. mouse anti-human antibody): Please note that strongly charged fluorescence dyes can interact with acidic and basic side chains of peptides. We thus recommend the use of neutral dyes or dyes with few charged functional groups (e.g. DyLight680, DyLight800, Cy3, Cy5) as well as pre-incubation of a peptide microarray with secondary antibodies to screen for possible background interactions. PEPperPRINT uses Pierce infrared dyes and the LI-COR Odyssey® Infrared Imaging System.
Peptide Microarray Immunoassay for Diagnostic Epitope Discovery (by Daniel Malamud)