PEPperCHIP® Trypanosoma Brucei Antigen Microarray

Profile antibody responses against 3 selected antigens of Trypanosoma Brucei.

The PEPperCHIP® Trypanosoma Brucei Antigen Microarray covers 3 selected antigens of Trypanosoma brucei translated into 1,522 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Each microarray chip contains two array copies for one-by-one assays. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery.

Request a Quote »

Microarray information

• 

Microarray content: Three selected antigens of Trypanosoma brucei: LiTat 1.3, LiTat 1.5, MiTat 1.4

• 

Peptide length and overlap: 15 aa peptides with peptide-peptide overlap of 14 aa (shift 1)

• 

Number of peptides / spots: 1,522 / 3,044 (peptides printed in duplicate)

• 

Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (31, 28 and 22 spots each control)

• 

Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 2 array copies per chip

• 

Accessories: compatible with 3/2 well PEPperCHIP® Incubation Tray

 

Sample information

• 

Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.

 

Estimated delivery time

• 

1-4 weeks for chip and optional add-on accessories

• 

6-8 weeks when requested with PEPperMAP® Services.

 

Documentation

• 

PEPperCHIP® Trypanosoma Brucei Antigen Microarray Material Data Sheet [download pdf]

• 

PEPperCHIP® Trypanosoma Brucei Antigen Microarray Peptide Map [download xlsx]

 

Learn how to perform the assay­­­

• 

PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]

• 

PEPperCHIP® Immunoassay Quickstart Guide [download pdf]

 

Steps

Materials and equipment

We recommend

Immunoassay

Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker

-

Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies

Buffers

PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software

-

*used in-house by PEPperPRINT

 

 

No scientific publications yet. Be the first one in publishing your data with this PEPperCHIP® Peptide Microarray.

PEPperCHIP® Trypanosoma Brucei Antigen Microarray

This is the tagline. Keep it short. Three sentences max.

<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

Request a Quote »

Ready to discuss your project?

Talk to one of our scientists to get started

Get in touch »Request a Quote »

Get everything you need for your PEPperCHIP® Peptide Microarray experiments

Explore related products and accessories