The PEPperCHIP® Peptidoglycan (PGN) Microarray is the first available tool to enable a high-throughput screening of antibody responses against 288 unique muropeptides, representing the most common amino acid variations of the peptide stem of bacterial cell walls with an N-terminal N-acetylmuramic acid (MucNAc). contains 288 different muropeptides printed in triplicate and enables the analysis of up to 16 samples per microarray. Moreover, the microarray contains FLAG positive control peptides.
The microarray can be used to:
Microarray information
• | Microarray content: 288 unique muropeptides, representing the most common amino acid variations of the peptide stem of bacterial cell walls with an N-terminal N-acetylmuramic acid (MucNAc). |
• | Peptide characteristics: The peptide stem of muropeptides is made of three to five amino acids including a number of less common amino acids like D-glutamic acid, homoserine, D-alanine, diaminobutanoic acid or D-glutamine with glycine and other interbridges between the peptide units. |
• | Number of peptides / spots: 288 / 891 (peptidoglycans printed at least in triplicate) |
• | Positive control: FLAG (DDDK) control peptides (105 spots) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 16 array copy per chip |
• | Accessories: compatible with 3/16 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Peptidoglycan Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Peptidoglycan Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
No scientific publications yet. Be the first one in publishing your data with this PEPperCHIP® Peptide Microarray.
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
