PEPperCHIP® Lupus Microarray

Profile auto-antibody responses against 28 selected Lupus associated antigens.

The PEPperCHIP® Lupus Microarray covers 28 selected lupus-associated autoantigens and 789 linear lupus epitopes including posttranslational modifications translated into 5,062 highly overlapping peptides. The microarray chip contains linear 15-mer peptides with a 14-mer overlap (shift 1) in duplicates for full epitope coverage. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.

The microarray can be used for epitope mapping, serum screening or biomarker discovery of autoantigens.

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Microarray information

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Microarray content: 28 selected Lupus autoantigens and additional 789 linear Lupus epitopes (3 - 50 aa) of the Immune Epitope Database including posttranslational modifications like citrulline and acetyllysine.

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Peptide lengths: 3 aa to 15 aa 

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Number of peptides / spots: 5,062 / 10,124 (peptides printed in duplicate)

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Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides  (28, 32 and 22 each control)

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Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip

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Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray

 

Sample information

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Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody.

 

Estimated delivery time

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1-4 weeks for chip and optional add-on accessories

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6-8 weeks when requested with PEPperMAP® Services.

Documentation

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PEPperCHIP® Lupus Microarray Material Data Sheet [download pdf]

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PEPperCHIP® Lupus Microarray Peptide Map [download xlsx]

 

Learn how to perform the assay­­­

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PEPperCHIP® Immunoassay Protocol [download pdf] [watch video]

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PEPperCHIP® Immunoassay Quickstart Guide [download pdf]

 

Steps

Materials and equipment

We recommend

Immunoassay

Incubation tray

PEPperCHIP® Incubation Tray*

Orbital shaker

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Blocking reagents

BSA, or Rockland Blocking Buffer*

Control antibodies

PEPperCHIP® Control Antibodies

Buffers

PBS, Tween 20

Labelled secondary antibodies

Rockland secondary antibodies*

Data acquisition

Microarray scanner

Innopsys InnoScan® 710/710-IR*
LI-COR Odyssey® scanners*

See a list of compatible scanners

Data analysis

Fluorescence spot intensity quantification software

MAPIX Analyzer by Innopsys

Spreadsheet software

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*used in-house by PEPperPRINT

 

 

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PEPperCHIP® Lupus Microarray

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<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>

<s>475.00 EUR</s>

Excl. tax and shipping costs.

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