The PEPperCHIP® Autoimmune Epitope Microarray covers 4,287 B-cell epitopes associated with autoimmune diseases including 286 citrullinated peptides and the corresponding arginine variants. All peptides are 15 amino acids long and printed in duplicates. Moreover, the microarray contains HA, polio and c-Myc positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery of autoantigens.
The PEPperCHIP® Autoimmune Epitope Microarray 3.0 provides a perfect platform for discovering autoantibodies, elucidating serum signatures of different patient groups and comparing the signatures with known epitopes and antigens from the Immune Epitope Database (IEDB).
Screen epitopes related to autoimmune diseases of
• | urogenital tract |
• | blood (e.g. antiphospholipid syndrome) |
• | cardiovascular system |
• | central nervous system (e.g. multiple sclerosis) |
• | endocrine and exocrine system (e.g. diabetes mellitus, Sjögren’s syndrome) |
• | eye, nose and throat |
• | gastrointestinal tract (e.g. Crohn’s disease) |
• | musculoskeletal system (e.g. rheumatoid arthritis) |
• | peripheral nervous system |
• | skin and connective tissue (e.g. vitiligo) |
• | systemic autoimmune disease (e.g. systemic lupus erythematosus) |
Microarray information
• | Microarray content: 4,287 epitopes of the Immune Epitope Database associated with autoimmune diseases incl. 286 citrullinated as well as corresponding arginine variant peptides |
• | Database epitope lengths: 3 aa - 51 aa |
• | Peptide length and overlap: 3 aa to 17 aa depending on the epitope length; longer epitopes were translated into overlapping 17 aa peptides |
• | Number of peptides / spots: 4,287 / 8,574 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT) and c-Myc (EQKLISEEDL) control peptides (40, 38 and 26 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Autoimmune Epitope Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Autoimmune Epitope Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
192 Disparities of B-Cell Type I Interferon Production and Responses in SLE.
Winn W. Chatham, Hui-Chen Hsu, John Mountz, Qi Wu, Alex Essman, Oluwagbemiga Ojo, Shanrun Liu, PingAr Yang, Bao Luo, and Jennie Hamilton. In Abstracts, A144.2-A145. Lupus Foundation of America, 2019. [doi.org/10.1136/lupus-2019-lsm.192]
Regulatory T-Cell Deficiency Leads to Pathogenic Bullous Pemphigoid Antigen 230 Autoantibody and Autoimmune Bullous Disease.
Stefanie Haeberle, Xiaoying Wei, Katja Bieber, Stephanie Goletz, Ralf J. Ludwig, Enno Schmidt, Alexander H. Enk, and Eva N. Hadaschik. Journal of Allergy and Clinical Immunology 142, no. 6 (December 2018): 1831-1842.e7. [doi.org/10.1016/j.jaci.2018.04.006]
General Approach for Tetramer-Based Identification of Autoantigen-Reactive B Cells: Characterization of La- and SnRNP-Reactive B Cells in Autoimmune BXD2 Mice.
Jennie A. Hamilton, Jun Li, Qi Wu, PingAr Yang, Bao Luo, Hao Li, John E. Bradley, et al. The Journal of Immunology 194, no. 10 (May 15, 2015): 5022–34. [doi.org/10.4049/jimmunol.1402335]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
