The PEPperCHIP® Allergen Epitope Microarray covers 4,022 linear allergen epitopes based on 223 different allergens across 90 different organisms. All peptides are 15 amino acids long and printed in duplicates. Moreover, the microarray contains HA, polio, c-Myc and IgE positive control peptides.
The microarray can be used for epitope mapping, serum screening or biomarker discovery.
The PEPperCHIP® Allergen Epitope Microarray can be used for the following applications
Identification of unknown allergic diseases in patient sera | |
Correlation of immediate hypersensitivity with IgE antibody profiles | |
Comparison of IgE responses with e.g. IgG or IgA profiles | |
Discrimination of antibody responses between multiple allergic diseases | |
A cross-correlation of allergen specific IgE responses with disease pathogenesis | |
Multiplexed analysis of allergen specific IgE antibody responses in human sera |
Advantages
Largest allergen epitope library on a single chip | |
Equivalent to more than 4,000 peptide ELISAs at once | |
Scientific background information available for each epitope with references to literature | |
Enables comparison of IgE responses with e.g. IgA and IgG profiles | |
Low sample consumption compared to nitrocellulose-assays | |
Most economical solution for high throughput peptide screening |
Microarray information
• | Microarray content: 4,022 linear allergen epitopes based on 223 different allergens referenced in the Immune Epitope Database |
• | Database epitope lengths: 5 aa - 53 aa |
• | Peptide length and overlap: 5 aa to 17 aa depending on the database epitope length; longer epitopes were translated into overlapping 17 aa peptides. |
• | Number of peptides / spots: 4,022 / 8,044 (peptides printed in duplicate) |
• | Positive control: HA (YPYDVPDYAG), polio (KEVPALTAVETGAT), c-Myc (EQKLISEEDL) and IgE (RHSTTQPRKTK) control peptides (26, 28, 18 and 32 spots each control) |
• | Size and format: 3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm, containing 1 array copy per chip |
• | Accessories: compatible with 3/1 well PEPperCHIP® Incubation Tray |
Sample information
• | Can be assayed with at least 20 µL serum/plasma or 10-20 µg/mL purified antibody. |
Estimated delivery time
• | 1-4 weeks for chip and optional add-on accessories |
• | 6-8 weeks when requested with PEPperMAP® Services. |
Documentation
• | PEPperCHIP® Allergen Epitope Microarray Material Data Sheet [download pdf] |
• | PEPperCHIP® Allergen Epitope Microarray Peptide Map [download xlsx] |
Learn how to perform the assay
• | PEPperCHIP® Immunoassay Protocol [download pdf] [watch video] |
• | PEPperCHIP® Immunoassay Quickstart Guide [download pdf] |
Steps | Materials and equipment | We recommend |
Immunoassay | Incubation tray | PEPperCHIP® Incubation Tray* |
Orbital shaker | - | |
Blocking reagents | BSA, or Rockland Blocking Buffer* | |
Control antibodies | PEPperCHIP® Control Antibodies | |
Buffers | PBS, Tween 20 | |
Labelled secondary antibodies | Rockland secondary antibodies* | |
Data acquisition | Microarray scanner | Innopsys InnoScan® 710/710-IR* |
Data analysis | Fluorescence spot intensity quantification software | MAPIX Analyzer by Innopsys |
Spreadsheet software | - |
*used in-house by PEPperPRINT
Immunization of Cats to Induce Neutralizing Antibodies against Fel d 1, the Major Feline Allergen in Human Subjects.
Franziska Thoms, Gary T. Jennings, Melanie Maudrich, Monique Vogel, Stefanie Haas, Andris Zeltins, Regina Hofmann-Lehmann, et al. Journal of Allergy and Clinical Immunology 144, no. 1 (July 2019): 193–203. [doi.org/10.1016/j.jaci.2019.01.050]
Combination of Two Epitope Identification Techniques Enables the Rational Design of Soy Allergen Gly m 4 Mutants.
Heide Havenith, Karolin Kern, Paul Rautenberger, Holger Spiegel, Michael Szardenings, Elke Ueberham, Jörg Lehmann, et al. Biotechnology Journal 12, no. 2 (February 2017): 1600441. [doi.org/10.1002/biot.201600441]
Purification and Characterization of Anacardium Occidentale (Cashew) Allergens Ana o 1, Ana o 2, and Ana o 3.
Marit Reitsma, Shanna Bastiaan-Net, Stefano Sforza, Johanna P. M. van der Valk, Roy van Gerth van Wijk, Huub F. J. Savelkoul, Nicolette W. de Jong, and Harry J. Wichers. Journal of Agricultural and Food Chemistry 64, no. 5 (February 10, 2016): 1191–1201. [doi.org/10.1021/acs.jafc.5b04401]
<s>For off-the-shelf products, see Standard chips and accessories checklist.xlsx for the product description that goes in this section.</s>
<s>475.00 EUR</s>
Excl. tax and shipping costs.
