by Laura Weber, PhD, Karlsruhe Institute of Technology, Germany
Wednesday, February 21, 2018 from 8:00-09:00 AM PST / 11:00-12:00 AM EST / 5:00-6:00 PM CET
Antibodies against pathogenic agents shield us from infections and are the basis of many diagnostic tests. Using peptide arrays, we investigated the antibody response upon vaccination with tetanus and the infection with Lyme disease with single amino acid resolution. The amino acid sequences of the tetanus toxin and the immunodominant VlsE antigen of B.burgdorferi were printed as overlapping peptides and incubated with patient sera.In a second step, interacting peptides were screened in an in-depth epitope substitution analysis to identify key amino acids that are indispensable for the binding of the respective antibody. Remarkably, our studies revealed in both cases that the antibody fingerprints are almost identical, if the same epitope is targeted by antibodies. The presented approach can be applied to:
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