In this webinar, we present a straightforward approach for the unambiguous validation of antibodies by combining protein and peptide microarrays
: An initial screening of antibodies against HuProt™ Human Proteome Microarrays with more than 20,000 human proteins enabled the determination of the target specificity and off-target binding on the protein level. For further validation of the antibody responses, we applied a multiplexed high resolution epitope mapping
of the top cross-reactions and the target protein by PEPperCHIP® Peptide Microarrays
. This resulted not only in the identification of the actual epitopes of the antibodies, but also provided more insight on the observed cross-reactions.