Systemic Lupus Erythematosus (SLE) is a multisystem autoimmune disease with mild to severe symptoms including a red rash, swollen joints and lymph nodes or chest pain. Although the suspected causes for SLE range from environmental triggers to vitamin D deficiency, a common serological feature is the production of autoantibodies. Despite a relatively small set of autoantibody targets, there is neither a single marker nor a marker panel used as a gold standard in SLE diagnosis.
For a better understanding of SLE-related autoantibody responses, we developed the novel PEPperCHIP® Lupus Microarray. The high density peptide microarraycovers28 lupus antigens translated into overlapping peptides for a highly multiplexed epitope mapping and 761 lupus epitopes of the Immune Epitope Database. The PEPperCHIP® Lupus Microarrayfurther contains epitopes with posttranslational modifications like citrulline and acetyllysine as well as the corresponding unmodified peptides as controls.
The PEPperCHIP® Lupus Microarrayenables the correlation of SLE -specific IgG, IgA or IgM autoantibody responses with pathogenesis, the search for differential antibody responses on the epitope level and hence the discovery of new prognostic SLE marker peptides.