In this application note, we describe the custom mapping of IgE epitopes of the cashew nut allergen Ana o 3 using human sera. The results demonstrate that PEPperCHIP® Peptide Microarrays are a valuable tool to obtain detailed knowledge of IgE epitopes in human serum that could be used for e.g. allergy diagnostics development. The peptide printing technology invented by PEPperPRINT allows synthesizing overlapping peptides with a shift of one amino acid directly on chip. This feature is the basis for a cost effective, customizable and fast solution for high resolution IgE epitope mapping.
- Download the New Application Note: IgE Epitope Mapping of the Cashe w Nut Allergen An a o 3
- Please download the raw data from our Application Note website.
In case the allergen is unclear, the PEPperCHIP® Allergen Epitope Microarray enables the multiplexed analysis of 4,022 linear allergen epitopes of the Immune Epitope Database in human sera to identify unknown allergic diseases in patient sera.
For more information on the epitope content of the new PEPperCHIP® Allergen Epitope Microarray, please download the material data sheet as well as the microarray layout scheme on the product website.