Identify and compare virus-specific antibody epitope recognition of different human coronavirus spike protein antigens in a single assay with the new PEPperCHIP® Pan-Corona Spike Protein Microarray. The chip contains the spike proteins of all seven human coronaviruses (SARS-CoV-2, SARS-CoV, MERS-CoVHCoV-HKU1, HCoV-OC43, HCoV-NL63, and HCoV-229E) translated into 4,564 individual peptides for a multiplexed epitope mapping.

 

Table 1. Overview of the different spike proteins included in the new PEPperCHIP® Pan-Corona Spike Protein Microarray. 

 SARS-CoV-2SARS-CoVMERS-CoVHCoV-HKU1HCoV-OC43HCoV-NL63HCoV-229E
UniProt IDP0DTC2P59594A0A140AYZ5U3NAI2P36334Q6Q1S2P15423
Protein sequence length1,2731,2551,3531,3561,3531,3561,173
Sequence homology       
vs. SARS-CoV-2-75.9%28.8%26.8%27.9%21.7%22.9%
vs. SARS-CoV75.9%-28.2%26.9%27.8%19.8%23.6%
vs. MERS-CoV28.8%28.2%-29.3%29.2%21.3%22.4%
vs. HCoV-HKU126.8%26.9%29.3%-62.5%22.4%22.1%
vs. HCoV-OC4327.9%27.8%29.2%62.5%-22.6%22.3%
vs. HCoV-NL6321.7%19.8%21.3%22.4%22.6%-54.8%
vs. HCoV-229E22.9%23.6%22.4%22.1%22.3%54.8%-

 

Screen purified antibodies, human or animal sera for coronavirus-specific spike protein antigen recognition. Analyze the cross-reactivity of diagnostic and therapeutic antibodies or compare patient-specific antibody response profiles for immunity or seroconversion monitoring. 

 

Applications: 

·    Serum antibody fingerprint analysis of IgG, IgM, and IgA antibody responses on the epitope level for IVD test development.

·    Immune monitoring of antibody responses in SARS-CoV-2 vaccination trials.

·    Epitope analysis of prognostic or neutralizing antibodies in patient sera for vaccine optimization and development.

·    Epitope identification and characterization of anti-SARS-CoV-2 antibodies.

 

 

 Antibody fingerprint or cross-reactivity analysis with the PEPperCHIP® Pan-Corona Spike Protein Microarray

 

Fig.1. Antibody fingerprint or cross-reactivity analysis workflow using the PEPperCHIP® Pan-Corona Spike Protein Microarray. Spike protein sequences of 7 human coronaviruses are translated into overlapping peptides and printed onto glass slides. Antibody samples, patient or animal sera are incubated on the chip followed by antibodiy binding to the corresponding spike protein epitopes. The resulting epitope profiles can be compared across different samples to determine antibody specificity and sensitivity, or to identify coronavirus-specfic target sequences for the development of diagnostic or therapeutic antibodies.

 

The new PEPperCHIP® Pan-Corona Spike Protein Microarray chip contains a single peptide array and is compatible with a 3/1 well PEPperCHIP® Incubation Tray. Each chip produced comes with HA and polio assay control peptide spots.

 

Downloads: Material Data Sheet / Microarray Layout

 

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Related products:

 

PEPperCHIP® SARS-CoV-2 Proteome Microarray 

PEPperCHIP® SARS Coronavirus Proteome Microarray (Material Data Sheet)

PEPperCHIP® SARS-CoV Antigen Microarray (Material Data Sheet)

PEPperCHIP® MERS-CoV Proteome Microarray (Material Data Sheet)

 


 

Further reading:

 

Distinct Early IgA Profile May Determine Severity of COVID-19 Symptoms: An Immunological Case Series.

Dahlke, Christine, Jasmin Heidepreim, Robin Kobbe, Rene Santer, Till Koch Anahita Fathi, My L. Ly, et al. Preprint. Infectious Diseases (except HIV/AIDS), April 17, 2020. [https://doi.org/10.1101/2020.04.14.20059733]

 

IgG and IgA Antibody Profiling with the PEPperCHIP® Infectious Disease Epitope Microarray. (Application note)

Discovery of serotype-specific epitope biomarkers for the diagnosis of Dengue virus infections. (Poster)

Profiling epitope-specific antibody responses against malaria utilizing high-density peptide arrays. (Poster)