Profile antibody responses against the most important wild type AAV capsid proteins with the PEPperCHIP® Pan-AAV Capsid Protein Microarray. The chip contains the sequences of 15 different capsid proteins converted into more than 5,000 overlapping peptides for high-resolution epitope data. Available with linear or cyclic constrained peptides for linear or conformational epitope mapping, the PEPperCHIP® Pan-AAV Capsid Protein Microarray can be used to analyze patient and animal sera as well as research and diagnostic anti-AAV antibodies on the epitope level.
· Anti-AAV antibody monitoring in patient sera before and after gene therapy
· Identification of cross-reacting anti-AAV antibodes
· Identification of pre-existing anti-AAV antibody responses in healthy individuals
· Fingerprint analysis of anti-AAV antibody responses in animal models
· Validation of research or diagnostic antibodies against AAVs
Fig. 1. Antibody fingerprinting workflow using the PEPperCHIP® Pan-AAV Capsid Protein Microarray. 15 different wild type AAV capsid proteins are converted into overlapping peptides and printed onto glass slides. Patient or animal serum is incubated on the microarray and antibodies present in the sample bind their corresponding epitopes on the microarray surface. The resulting response profiles can be compared across different samples to identify and monitor antibody reactivity against AAVs used in gene therapy research and development.
Both linear and cyclic constrained PEPperCHIP® Pan-AAV Capsid Protein Microarrays contain a single peptide array and is compatible with a 3/1 well the PEPperCHIP® Incubation Tray. Each chip produced comes with built-in HA and polio assay control peptide spots.