PEPperCHIP® Custom Peptide Microarrays
Specifications
From single antigens translated into overlapping peptides to whole virus proteomes – PEPperCHIP® Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner.
| Peptide Synthesis: | On-chip using Fmoc chemistry and routine double coupling steps |
| Microarray Format: | Standard slide format (3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm) |
| Number of Peptides: | From 130 to 5,500 peptides printed in duplicate |
| Arrays Copies per Chip: | From single arrays to 16 arrays per chip depending on the number of peptides |
| Array Content: | Custom protein-derived overlapping peptides, peptide collections, substitution scans etc. |
| Available Amino Acids: | L- and D-amino acids, citrulline and other amino acids on request |
| Peptide Length: | Routine synthesis of up to 15mer peptides, longer peptides on request |
| Control Peptides: | HA (YPYDVPDYAG) and polio (KEVPALTAVETGAT) epitopes, other controls on request |
We support you with your optimal peptide array design and project setup. Please contact us to discuss your requirements.
Applications
Input Data & Sample Material
- 10-20 µl serum/plasma or 10-20 µg purified antibody
- Protein sequences (e.g. FASTA format or UniProt ID) or peptide collections (Excel, Word or text files)
Analysis
PEPperCHIP® Peptide Microarray analysis is straightforward and can be done in your own lab or by PEPperPRINT experts within a PEPperMAP® Service Offer. Further information about lab requirements for peptide microarray analysis can be found here.
Timeline
From peptide array design to production and quality control, it takes about 3 weeks.
Advantages
- Highest spot densities and numbers of peptides per chip
- Widely unlimited peptide content flexibility
- Adjustment of peptide microarray layouts according to your specifications
- Cost-effective solutions without excessive and expensive peptide pre-synthesis
- Multiple peptide array copies per chip for one-by-one assays
- Outstanding signal-to-noise ratios due to custom slide coatings
- Straighforward handling and analysis
- Comprehensive support before, during and after purchase
Further Information
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