PEPperCHIP® Custom Peptide Microarrays

Specifications

From single antigens translated into overlapping peptides to whole virus proteomes – PEPperCHIP® Custom Peptide Microarrays can be fully tailored in a uniquely cost-effective manner.


  Peptide Synthesis:  On-chip using Fmoc chemistry and rountine double coupling steps
  Microarray Format:  Standard slide format (3'' x 1'', 75.4 mm x 25.0 mm x 1.0 mm)
  Number of Peptides:  From 100 to 4,300 peptides printed in duplicate
  Arrays Copies per Chip:  From single arrays to 16 arrays per chip depending on the number of peptides
  Array Content:  Custom protein-derived overlapping peptides, peptide collections, substitution scans etc.
  Available Amino Acids:  L- and D-amino acids, citrulline and other amino acids on request
  Peptide Length:  Rountine synthesis of up to 15mer peptides, longer peptides on request
  Control Peptides:  Standard HA (YPYDVPDYAG) and Flag (DYKDDDDKAS) tags, other controls on request

 

We support you with your optimal peptide array design and project setup. Please contact us to discuss your requirements.

 

 PEPperCHIP® Custom Peptide Microarrays

Applications



Input Data & Sample Material

  • 10-20 µl serum/plasma or 10-20 µg purified antibody
  • Protein sequences (e.g. FASTA format or UniProt ID) or peptide collections (Excel, Word or text files)



Analysis

PEPperCHIP® Peptide Microarray analysis is straightforward and can be done in your own lab or by PEPperPRINT experts within a PEPperMAP® Service Offer. Further information about lab requirements for peptide microarray analysis can be found here.

 

 Array Layout

Timeline

From peptide array design to production and quality control, it takes about 3 weeks.

 

 

Advantages

  • Highest spot densities and numbers of peptides per chip
  • Widely unlimited peptide content flexibility
  • Adjustment of peptide microarray layouts according to your specifications
  • Cost-effective solutions without excessive and expensive peptide pre-synthesis
  • Multiple peptide array copies per chip for one-by-one assays
  • Outstanding signal-to-noise ratios due to custom slide coatings
  • Straighforward handling and analysis
  • Comprehensive support before, during and after purchase


 

Further Information

 

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