The standard slide format facilitates application of PEPperCHIP® Peptide Microarrays with standard lab infrastructures and most microarray scanners. The following recommended PEPperCHIP® accessories further support the straightforward use of our state-of-the-art peptide microarray solutions to ensure optimized assay conditions and best data quality.
PepSlide® Analyzer is an intuitive and easy-to-handle software solution for peptide microarray image analysis and supports quantification of microarray data, the interpretation of various peptide contents (e.g. permutation scans or overlapping antigen-derived peptides) as well as statistical data mining tools. The software is adjusted to the PEPperCHIP® Peptide Microarray platform and links microarray design files to content information and quantitative microarray data for data analysis. PepSlide® Analyzer further provides the best representation of PEPperCHIP® spot morphologies and is, thus, more than a cost-effective alternative to GenePix® Pro.
PepSlide® Analyzer ensure proper spot recognition and intensity quantification based on standard scanner tiff files (left); the linkage between microarray design and content as well as quantified microarray data provides a straightforward approach for data evaluation and interpretation.
The main features and specifications of PepSlide® Analyzer are summarized below:
- Efficient tools for image processing, array alignment and spot finding
- Use microarray scanners‘ tiff images and load design data from GAL files or PepSlide® PSF files
- Compatibility with most microarray scanners
- Perfectly adapted to the PEPperCHIP® platform
- Full compatibility with Excel
- Recognition of custom spot sizes and morphologies
- State of the art background correction
- Cost-effective alternative to GenePix® Pro
- Available for Windows and Mac OS X platforms
The quick start guide and the manual of PepSlide® Analyzer are available in the Download Section. PepSlide®Analyzer can be evaluated as free trial with full functionality and support for 14 days.
PEPperCHIP® Incubation Tray
PEPperCHIP® Incubation Trays enable one-by-one assays with multiple array copies per PEPperCHIP® microarray. The incubation trays are available in well formats 3/1, 3/2, 3/3, 3/4, 3/5, 3/16 and 3/(3-4-5) (slides/wells), and are, thus, compatible with all available PEPperCHIP® Peptide Microarray layouts with 1-5 and 16 array copies per chip.
PEPperCHIP® Incubation Trays are fully reusable with minimal sample consumption in line with immunoassays. The recommended shaking incubation is also supported (see PEPperCHIP® Immunoassay Protocol) to provide optimal assay qualities and data outcome.
PEPperCHIP® Staining Kit
PEPperCHIP® Peptide Microarrays contain HA- and FLAG-tags as standard controls, usually arranged as frame around the main content or for content segmentation.
According to the PEPperCHIP® Immunoassay Protocol, we recommend the staining of these microarray controls after the main assays by incubation with suited anti-HA and anti-FLAG antibodies. The PEPperCHIP® Staining Kit provides both antibodies either labeled with Cy3/Cy5 for standard microarray scanners (wavelengths 532 nm and 635 nm), or with infrared dyes DyLight 680/DyLight 800 for microarray scanners like the LI-COR Odyssey scanners (wavelengths 700 nm and 800 nm).
If used at the recommended dilution of 1:1000, the PEPperCHIP® Staining Kit provides conjugated anti-HA and anti-FLAG antibodies for control assays with up to five PEPperCHIP® Peptide Microarrays.
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