PEPperCHIP® Human Epitome Microarray

For the very first time, the new PEPperCHIP® Human Epitome Microarray covers all linear human B-cell epitopes of the Immune Epitope Database.

 

The 28,895 different peptides of the PEPperCHIP® Human Epitome Microarray are based on 23,163 database epitopes of 2,542 different proteins and 468 different organisms. Sorted by disease states, the PEPperCHIP® Human Epitome Microarray covers the following indications:

 

  • 8,504 epitopes of infectious diseases
  • 3,808 epitopes of autoimmune diseases including 301 citrullinated peptides
  • 3,451 epitopes of allergens
  • 199 cancer epitopes
  • 995 epitopes of other diseases
  • 1,481 epitopes of healthy controls
  • 4,661 epitopes of the most common vaccines

 

Applications

The PEPperCHIP® Human Epitome Microarray enables the high throughput screening of serum and plasma antibody responses against all linear human B-cell epitopes of the Immune Epitope Database: This corresponds to 28,895 peptide ELISA in a single assay with less than 50 µl sample need.

PEPperCHIP® Human Epitome Microarray

Microarray scan of a PEPperCHIP® Human Epitome Microarray with IgG (red) and IgM (green) response profiles from patient serum (left); IgG and IgM hit lists are directly linked with Immune Epitope Database entries and literature as well as the corresponding antigen.


The PEPperCHIP® Human Epitome Microarray covers the following applications:


  • epitome-wide antibody screening
  • differentiation between IgG and IgM or IgA response profiles
  • high throughput serum biomarker discovery
  • identification of prognostic epitopes
  • cross-reactivity analysis of mono- and polyclonal antibodies
  • patient stratification
  • investigation of links between autoimmune and infectious diseases
  • cross-reactivity analysis of therapeutic and diagnostic antibodies

 

The new PEPperCHIP® Human Epitome Microarray is intended for research use. For more information on the epitope content, please download the material data sheet as well as the microarray layout scheme.

 

 


Analysis

Due to the discovery microarray format and the resulting large data sets, PEPperCHIP® Human Epitome Microarrays are exclusively provided in combination with a PEPperMAP® Services.

 

 

Sample Need

  • 50 µl serum or 50 µg purified antibody


 

Timeline

The PEPperCHIP® Human Epitome Microarray is usually available within 1-2 weeks. We ask to ship the sample(s) within this period. The assays will take 1-2 weeks followed by another week for data quantification, evaluation and reporting. A custom screening service based on a PEPperCHIP® Human Epitome Microarray, hence, requires 3-5 weeks from order confirmation to delivery of the scientific report.

 

 

Advantages

  • largest human epitope library available on peptide microarray
  • equivalent to more than 28,000 peptide ELISAs at once
  • most economical solution for high throughput peptide screening
  • outstanding signal-to-noise ratios due to proprietary slide coatings
  • comprehensive analysis by PEPperPRINT's peptide microarray experts
  • data evaluation and reporting on a scientific level


 

Further Information

 

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